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Evidence of frequent integration of non-T-DNA vector backbone sequences in transgenic strawberry plant

Paper ID Volume ID Publish Year Pages File Format Full-Text
22076 43252 2006 3 PDF Available
Title
Evidence of frequent integration of non-T-DNA vector backbone sequences in transgenic strawberry plant
Abstract

We have studied the occurrence of the integration of non-T-DNA sequences in transgenic strawberry plants obtained through Agrobacterium inoculation. DNA from these plants was subjected to PCR amplification of the sequence of the gene trfA, which is located outside the T-DNA. The percentage of trfA-positive plants varied from 40% to 90%, with a mean of 65.7%. Backbone sequences were confirmed by Southern blot analysis.

Keywords
Agrobacterium tumefaciens; Fragaria x ananassa; T-DNA transfer; genetic transformation
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Evidence of frequent integration of non-T-DNA vector backbone sequences in transgenic strawberry plant
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 6, June 2006, Pages 508–510
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us