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Reconstruction of cartilage tissue using scaffold-free organoid culture technique

Paper ID Volume ID Publish Year Pages File Format Full-Text
22080 43253 2008 4 PDF Available
Title
Reconstruction of cartilage tissue using scaffold-free organoid culture technique
Abstract

We applied the scaffold-free culture method to chondrocytes and attempted to reconstitute articular cartilage grafts. Primary rat costal chondrocytes were immobilized into hollow fibers by centrifugation at a density of 3×108 cells/cm3 to induce the formation of cylindrical-shaped multicellular aggregates (organoids) and cultured for one month. The organoids were evaluated by histological and gene expression analyses. Chondrocytes formed cylindrical organoids in hollow fibers (HFs). Histochemical analysis revealed the accumulation of a cartilage extracellular matrix (collagen and proteoglycan) around cells in the lumen of HFs with culture time, forming a low-cellular-density tissue similar to native cartilage by day 28. Furthermore, in contrast to that in traditional monolayer culture, the organoid maintained the gene expression of the cartilage extracellular matrix (type II collagen, aggrecan) for one month of culture. In conclusion, our organoid formation method was effective in producing a cartilage-like tissue. This result suggests that the technique may be applicable to the development of an articular cartilage graft.

Keywords
chondrocytes; articular cartilage; scaffold-free; organoid; three-dimensional culture; hollow fiber; gene expression
First Page Preview
Reconstruction of cartilage tissue using scaffold-free organoid culture technique
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 5, May 2008, Pages 450–453
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering