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High throughput and exhaustive analysis of diverse lipids by using supercritical fluid chromatography-mass spectrometry for metabolomics

Paper ID Volume ID Publish Year Pages File Format Full-Text
22082 43253 2008 10 PDF Available
Title
High throughput and exhaustive analysis of diverse lipids by using supercritical fluid chromatography-mass spectrometry for metabolomics
Abstract

We have developed an analytical system that enables the simultaneous rapid analysis of lipids with varied structures and polarities through the use of supercritical fluid chromatography-mass spectrometry (SFC-MS). The separation conditions for SFC (column, modifier, back pressure, etc.) and the detection conditions for mass spectrometry (ionization method, parameters, etc.) were investigated to develop a simultaneous analytical method for lipid mixtures that included phospholipids, glycolipids, neutral lipids, and sphingolipids. When cyanopropylated silica gel-packed column was used for the separation, all lipids were successfully detected and the analysis time was less than 15 min. The use of an octadecylsilylated column resulted in separation, which was dependent on the differences in the unsaturation of the fatty acid side chains and isomer separation. This system is a powerful tool for studies on lipid metabolomics because it is useful not only as a fingerprinting method for the screening of diverse lipids but also for the detailed profiling of individual components.

Keywords
lipid; metabolomics; metabolome; lipidomics; supercritical fluid chromatography; mass spectrometryCB, cerebroside; Cer, ceramide; DG, diacylglycerol; DGDG, digalactosyldiacylglycerol; LC, liquid chromatography; LPC, lysophosphatidylcholine; MGDG, monogala
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High throughput and exhaustive analysis of diverse lipids by using supercritical fluid chromatography-mass spectrometry for metabolomics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 5, May 2008, Pages 460–469
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us