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Application of elastic salmon collagen gel to uniaxial stretching culture of human umbilical vein endothelial cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
22095 43253 2008 4 PDF Available
Title
Application of elastic salmon collagen gel to uniaxial stretching culture of human umbilical vein endothelial cells
Abstract

In this study, we investigated the potential of an elastic salmon collagen gel (e-gel) for use as stretching culture scaffold. First, human umbilical vein endothelial cells (HUVECs) were cultured on the e-gel under static condition, and their growth was evaluated by DNA content measurement, MTT test, and scanning electron microscopy. The results demonstrated steady increases in cell number with culture time. Next, HUVECs were cultured on the e-gel under static condition for 2 d, then uniaxially stretched at a constant frequency (10% elongation at 1 Hz). After the stretching culture for 2 h, the cells oriented perpendicularly to the stretch direction. Moreover, the interleukin-6 and interleukin-8 productions of the cells significantly increased under the stretch condition compared with those under the static condition. These results were in good agreement with the published data in which an elastic silicone membrane was used as a scaffold. In conclusion, the e-gel can be used for stretching culture for vascular tissue engineering.

Keywords
elastic collagen; salmon collagen; scaffold; stretching culture; endothelial cells
First Page Preview
Application of elastic salmon collagen gel to uniaxial stretching culture of human umbilical vein endothelial cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 5, May 2008, Pages 554–557
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering