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Cell behavior analysis to evaluate proliferative potentials of human lymphocytes expanded and activated for therapeutic use

Paper ID Volume ID Publish Year Pages File Format Full-Text
22098 43253 2008 4 PDF Available
Title
Cell behavior analysis to evaluate proliferative potentials of human lymphocytes expanded and activated for therapeutic use
Abstract

The cluster designation 3-lymphokine activated killer (CD3-LAK) culture was conducted using human lymphocytes obtained from different donors. It was found that donor-dependent variances existed in terms of lag time and minimum doubling time, which were process parameters for comprehending the proliferative potentials of cells in an early phase with weak growth and in a subsequent phase with active growth, respectively. To correlate these variances with culture performances, cellular behaviors were estimated by constructing a custom-made observation tool that can capture and process images of culture surfaces. The tool enabled us to determine time-lapse changes in an average projected cell area and the frequency of cell aggregates during the culture in a noninvasive manner. It was found that linear relationships were obtained both between lag time and average projected cell area, and between minimum doubling time and formation rate of cell aggregates, irrespective of culture performance fluctuation depending on each donor state. It is concluded that the developed tool is helpful for operating CD3-LAK culture while monitoring the state of human lymphocytes.

Keywords
cell therapy; human lymphocytes; image analysis; cellular behaviors; cell aggregates
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Cell behavior analysis to evaluate proliferative potentials of human lymphocytes expanded and activated for therapeutic use
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 5, May 2008, Pages 566–569
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us