Modulation of talA gene in pentose phosphate pathway for overproduction of poly-β-hydroxybutyrate in transformant Escherichia coli harboring phbCAB operon
The talA gene encoding transaldolase, the key enzyme in the nonoxidative pentose phosphate pathway, was amplified in a transformant Escherichia coli harboring the phbCAB operon to shift the metabolic flux of the hexose mono-phosphate shunt to the odd-ball biosynthesis pathway for poly-β-hydroxybutyrate overproduction. The PHB content in the transformant E. coli coharboring the phbCAB operon and talA gene increased from 28.2% to 52.3%, and the retarded cell growth was overcome. This increase seems to be mainly due to the concomitant supplies of the intermediates NADPH and acetyl-CoA, which are from the activated pentose phosphate pathway through the modulation of the talA gene and from the Embden–Meyerhof pathway.
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 3, September 2006, Pages 237–240