fulltext.study @t Gmail

Modulation of talA gene in pentose phosphate pathway for overproduction of poly-β-hydroxybutyrate in transformant Escherichia coli harboring phbCAB operon

Paper ID Volume ID Publish Year Pages File Format Full-Text
22115 43254 2006 4 PDF Available
Title
Modulation of talA gene in pentose phosphate pathway for overproduction of poly-β-hydroxybutyrate in transformant Escherichia coli harboring phbCAB operon
Abstract

The talA gene encoding transaldolase, the key enzyme in the nonoxidative pentose phosphate pathway, was amplified in a transformant Escherichia coli harboring the phbCAB operon to shift the metabolic flux of the hexose mono-phosphate shunt to the odd-ball biosynthesis pathway for poly-β-hydroxybutyrate overproduction. The PHB content in the transformant E. coli coharboring the phbCAB operon and talA gene increased from 28.2% to 52.3%, and the retarded cell growth was overcome. This increase seems to be mainly due to the concomitant supplies of the intermediates NADPH and acetyl-CoA, which are from the activated pentose phosphate pathway through the modulation of the talA gene and from the Embden–Meyerhof pathway.

Keywords
transaldolase; talA gene; pentose phosphate pathway; transformant Escherichia coli; phbCAB operon; metabolic engineering; poly-β-hydroxybutyrate (PHB) biosynthesis
First Page Preview
Modulation of talA gene in pentose phosphate pathway for overproduction of poly-β-hydroxybutyrate in transformant Escherichia coli harboring phbCAB operon
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 3, September 2006, Pages 237–240
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering