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Monitoring of Phytopathogenic Ralstonia solanacearum Cells Using Green Fluorescent Protein-Expressing Plasmid Derived from Bacteriophage ϕRSS1

Paper ID Volume ID Publish Year Pages File Format Full-Text
22121 43255 2007 6 PDF Available
Title
Monitoring of Phytopathogenic Ralstonia solanacearum Cells Using Green Fluorescent Protein-Expressing Plasmid Derived from Bacteriophage ϕRSS1
Abstract

A green fluorescent protein (GFP)-expressing plasmid was constructed from a filamentous bacteriophage ϕRSS1 that infects the phytopathogen Ralstonia solanacearum. This plasmid designated as pRSS12 (4.7 kbp in size) consists of an approximately 2248 bp region of the ϕRSS1 RF DNA, including ORF1-ORF3 and the intergenic region (IG), and a Km cassette in addition to the GFP gene. It was easily introduced by electroporation and stably maintained even without selective pressure in strains of R. solanacearum of different races and biovars. Strong green fluorescence emitted from pRSS12-transformed bacterial cells was easily monitored in tomato tissues (stem, petiole, and root) after infection as well as from soil samples. These results suggest that pRSS12 can serve as an easy-to-use GFP-tagging tool for any given strain of R. solanacearum in cytological as well as field studies.

Keywords
bacterial monitoring; green fluorescent protein (GFP); phage-derived plasmid; bacterial wilt; Ralstonia solanacearum
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Monitoring of Phytopathogenic Ralstonia solanacearum Cells Using Green Fluorescent Protein-Expressing Plasmid Derived from Bacteriophage ϕRSS1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 6, December 2007, Pages 451–456
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us