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Convenient Transformation of Anamorphic Basidiomycetous Yeasts Belonging to Genus Pseudozyma Induced by Electroporation

Paper ID Volume ID Publish Year Pages File Format Full-Text
22132 43255 2007 4 PDF Available
Title
Convenient Transformation of Anamorphic Basidiomycetous Yeasts Belonging to Genus Pseudozyma Induced by Electroporation
Abstract

A convenient procedure for carrying out transformation by electroporation was optimized for the genus Pseudozyma. Successful transformation was achieved using a plasmid, pUXV1, that confers resistance to hygromycin B; the maximum transformation efficiency was 48 transformants/μg of plasmid DNA. Transformants of Pseudozyma antarctica T-34 expressing a green fluorescent protein were obtained by the procedure.

Keywords
Pseudozyma; yeast; transformation; electroporation; green fluorescent protein (GFP); hygromycin B
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Convenient Transformation of Anamorphic Basidiomycetous Yeasts Belonging to Genus Pseudozyma Induced by Electroporation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 6, December 2007, Pages 517–520
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us