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Generation and characterization of islet cell tumor in pTet-on/pTRE-SV40Tag double-transgenic mice model

Paper ID Volume ID Publish Year Pages File Format Full-Text
22148 43257 2007 8 PDF Available
Title
Generation and characterization of islet cell tumor in pTet-on/pTRE-SV40Tag double-transgenic mice model
Abstract

A line of double-transgenic mice that develop neoplasms arising primarily in the pancreas was established. In these mice, the oncogene SV40 T antigen (Tag) was detected in the pancreas with and without the control of Tet-on system. The transgenic mice that developed pancreatic tumors as early as 20 weeks of age showed hypoglycemia on a blood glucose test. Pathological and immunohistochemical characterizations demonstrated that the tumors belonged to neuroendocrine neoplasms arising from pancreatic islets. A change in IGFs/IGF-1R signaling pathway was detected using real-time PCR analysis. A potential association between the IGFs/IGF-1R system and SV40Tag was studied to further explain the cancerogenesis of the double-transgenic mice by Western blot analysis and immunoprecipitation experiments. The results suggest that a Tag transgenic mice model could be used to study the molecular mechanism of the tumorigenesis of islets.

Keywords
pTet-on/pTRE-SV40Tag; double-transgenic mice; islet cell tumors; IGFs/IGF-1R pathway; hypoglycemia
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Generation and characterization of islet cell tumor in pTet-on/pTRE-SV40Tag double-transgenic mice model
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 1, July 2007, Pages 14–21
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us