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Cloning, characterization and expression analysis of calcium channel β subunit from pearl oyster (Pinctada fucata)

Paper ID Volume ID Publish Year Pages File Format Full-Text
22153 43257 2007 8 PDF Available
Title
Cloning, characterization and expression analysis of calcium channel β subunit from pearl oyster (Pinctada fucata)
Abstract

The absorption, transport and localization of calcium underlie the basis of biomineralization, and Ca2+ entry into epithelial cell is the primary step in shell formation. However, the related mechanism of Ca2+ transport is poorly documented at the gene or protein level. L-type voltage-dependent calcium channels may be involved in calcium transport for biomineralization in some marine invertebrates. In this study, a full-length cDNA of a voltage-dependent calcium channel β subunit from Pinctada fucata (PCaβ) was cloned, and its amino acid sequence was deduced. PCaβ shared 51%–67% apparently sequence identity with voltage-dependent calcium channel β subunits from other species. However, PCaβ was much shorter than other voltage-dependent calcium channel β subunits particularly at the carboxyl terminus, indicating that it is likely a truncated β subunit isoform. Semi-quantitative RT-PCR analysis showed that PCaβ was expressed in all the tested tissues and that it had a higher expression level in the gill tissue and hemolymph than in other tissues, suggesting that L-type voltage-dependent calcium channels are responsible for Ca2+ absorption in the gill and Ca2+ entry into hemocytes. In the mantle, PCaβ mRNA was predominantly expressed in the inner and middle folds of the mantle epithelium, suggesting that L-type voltage-dependent calcium channels are involved in Ca2+ absorption from the ambient medium in the mantle. All these results suggest that voltage-dependent calcium channels are involved in Ca2+ uptake and transport during oyster biomineralization.

Keywords
calcium channel; β subunit; cDNA cloning; expression analysis; biomineralization; Pinctada fucata; mollusk
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Cloning, characterization and expression analysis of calcium channel β subunit from pearl oyster (Pinctada fucata)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 1, July 2007, Pages 47–54
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us