fulltext.study @t Gmail

Identification of enzyme responsible for erythritol utilization and reaction product in yeast Lipomyces starkeyi

Paper ID Volume ID Publish Year Pages File Format Full-Text
22162 43258 2006 6 PDF Available
Title
Identification of enzyme responsible for erythritol utilization and reaction product in yeast Lipomyces starkeyi
Abstract

We have identified the enzyme responsible for erythritol utilization and its reaction product in the yeast Lipomyces starkeyi CBS 1807. The enzyme, a polyol dehydrogenase requiring NAD+ as a coenzyme, was induced by erythritol in this yeast. We confirmed that the enzyme product was l-erythrulose by MS, NMR, and polarimeter analyses, meaning that we clarified the first step of erythritol utilization in yeasts for the first time. In the case of the oxidative reaction, d-threitol, (2R,3R)-2,3-butanediol, and erythritol were much better substrates than 21 other polyols tested. These three substrates are tetroses and have an R configuration at C-3, and whose third carbon results in easiest oxidation in this enzyme. The research of the substrate specificity in the reductive reaction demonstrated that l-erythrulose and dihydroxyacetone were better substrates, that d-acetoin was inactive and l-erythrose (aldose) was slightly active.

Keywords
dehydrogenase; erythritol; erythrulose; polyol; yeast
First Page Preview
Identification of enzyme responsible for erythritol utilization and reaction product in yeast Lipomyces starkeyi
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 4, April 2006, Pages 303–308
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us