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Efficient biosynthesis of d-allose from d-psicose by cross-linked recombinant l-rhamnose isomerase: Separation of product by ethanol crystallization

Paper ID Volume ID Publish Year Pages File Format Full-Text
22168 43258 2006 6 PDF Available
Title
Efficient biosynthesis of d-allose from d-psicose by cross-linked recombinant l-rhamnose isomerase: Separation of product by ethanol crystallization
Abstract

Mass production of a rare aldohexose d-allose from d-psicose was achieved in a batch reaction by crude recombinant l-rhamnose isomerase (l-RhI) cross-linked with glutaraldehyde. The d-psicose substrate was, in turn, mass produced from a naturally abundant ketohexose d-fructose by immobilized recombinant d-tagatose 3-epimerase (d-TE). At an equilibrium state, 25% of d-psicose was isomerized to d-allose, that is, 25 g of d-allose was obtained from 100 g of d-psicose. The d-allose product was easily separated and crystallized from the reaction mixture that contains 25% d-allose, 8% d-altrose and 67% d-psicose using ethanol. Empirically, approximately 338 g, that is, 90% of a theoretical overall yield for the purification of pure d-allose crystals was produced from 1.5 kg of d-psicose within 30 d using a constructed bioreactor. The cross-linked enzyme had an operative half-life of two months after repeated usages.

Keywords
rare sugar; d-psicose; d-allose; l-rhamnose isomerase
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Efficient biosynthesis of d-allose from d-psicose by cross-linked recombinant l-rhamnose isomerase: Separation of product by ethanol crystallization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 4, April 2006, Pages 340–345
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
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Any Questions? feel free to contact us