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Functional expression of streptococcal galactosyltransferase in baculovirus/insect cell expression system

Paper ID Volume ID Publish Year Pages File Format Full-Text
22173 43258 2006 4 PDF Available
Title
Functional expression of streptococcal galactosyltransferase in baculovirus/insect cell expression system
Abstract

The cpsIaJ gene of Streptococcus agalactiae type Ia codes for β-1,4-galactosyltransferase. In this study, the functional expression of His-tagged CpsIaJ in a baculovirus expression system was performed, because the efficient functional expression of this enzyme in Escherichia coli had been unsuccessful. Using a partially purified enzyme preparation, we found that the enzyme had a restricted substrate specificity and that the entire structure of the substrate GlcNAcβ1-3Galβ1-4Glc was required for the activity. Furthermore, mutations in a conserved DXD motif caused the loss of the enzyme's activity.

Keywords
galactosyltransferase; Streptococcus agalactiae; baculovirus; protein expression
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Functional expression of streptococcal galactosyltransferase in baculovirus/insect cell expression system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 4, April 2006, Pages 372–375
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us