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Microbial production of xylitol from l-arabinose by metabolically engineered Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
22178 43259 2009 6 PDF Available
Title
Microbial production of xylitol from l-arabinose by metabolically engineered Escherichia coli
Abstract

An Escherichia coli strain, ZUC99(pATX210), which can produce xylitol from l-arabinose at a high yield, has been created by introducing a new bioconversion pathway into the cells. This pathway consists of three enzymes: l-arabinose isomerase (which converts l-arabinose to l-ribulose), d-psicose 3-epimerase (which converts l-ribulose to l-xylulose), and l-xylulose reductase (which converts l-xylulose to xylitol). The genes encoding these enzymes were cloned behind the araBAD promoter in tandem so that they were polycistronically transcribed from the single promoter, like an operon. Expression of the recombinant enzymes in the active form was successfully achieved in the presence of l-arabinose. A xylitol production profile of the recombinant strain was evaluated by shake-flask fermentation. ZUC99(pATX210) produced 2.6 g/l xylitol using 4.2 g/l l-arabinose with a xylitol yield of 0.62 g/g l-arabinose in 36 h. It was determined that utilization of glycerol as a co-substrate significantly improved xylitol production and yield. In the presence of 11.8 g/l glycerol, ZUC99(pATX210) produced 9.7 g/l xylitol from 10.5 g/l l-arabinose with a xylitol yield of 0.92 g/g l-arabinose in 36 h. ZUC99(pATX210) also exhibited efficient conversion in fermentor experiments with 1 l medium containing l-arabinose and glycerol. The strain produced 14.5 g/l xylitol from 15.2 g/l l-arabinose with a xylitol yield of 0.95 g/g l-arabinose in 30 h.

Keywords
Xylitol; l-Arabinose; Escherichia coli; Metabolic engineering; Cofactor regeneration
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Microbial production of xylitol from l-arabinose by metabolically engineered Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 5, May 2009, Pages 506–511
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us