fulltext.study @t Gmail

Investigation of inclusion body formation in recombinant Escherichia coli with a bioimaging system

Paper ID Volume ID Publish Year Pages File Format Full-Text
22179 43259 2009 4 PDF Available
Title
Investigation of inclusion body formation in recombinant Escherichia coli with a bioimaging system
Abstract

A bioimaging system using giant protoplasts of recombinant Escherichia coli was developed for examining conditions affecting the formation of inclusion bodies. After the giant protoplasts were prepared, isopropyl-β-d-thiogalactopyranoside (IPTG) was used to induce expression of the blue fluorescence protein (BFP) gene. Fluorescence microscopy revealed that two types of fluorescence were emitted; one by soluble BFP and the other by aggregated BFP. As a result, the parameters that influence the formation of inclusion bodies can be examined by observing the fluorescence emitted by BFP, in real-time, with a fluorescence microscope. It was found that the aggregated BFP decreased with decreasing cultivation temperature, and the formation of inclusion bodies increased with decreased pH and increased IPTG concentrations. In addition, the aggregated form of BFP emitted fluorescence, indicating that BFP inclusion bodies might remain, at least partially, in a properly folded native-like form.

Keywords
Escherichia coli; Giant protoplast; Inclusion body; Induction; Blue fluorescence protein
First Page Preview
Investigation of inclusion body formation in recombinant Escherichia coli with a bioimaging system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 5, May 2009, Pages 512–515
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us