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Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
22187 43259 2009 4 PDF Available
Title
Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes
Abstract

Hepatocyte organoids have an in vivo-like cell morphology and maintain cell viability and function in vitro. On the other hand, the oxygen supply to hepatocytes is sometimes limited in the core of organoids that are more than 100 μm in thickness. In this study, we designed and examined a new bioreactor using sheet-shaped organoids (organoid-sheets) in which the thickness was controlled to prevent hepatocyte death in the core of organoid due to limitation of oxygen supply. The cell culture space consisted of stacked organoid formation spaces and medium flow channels. Each space was separated by flat porous polycarbonate membranes, and the organoid thickness was controlled at 100 μm with a stainless steel spacer. Freshly isolated hepatocytes (7.0 × 107) were immobilized in the bioreactor, yielding a cell density of 4.5 × 107 cells/cm3-bioreactor. Of the five flow rates tested (1.0, 5.0, 10, 20, and 50 mL/min), the bioreactor with the 10 mL/min had the highest ammonia removal and albumin secretion activities for at least 14 days. In conclusion, a new bioreactor controlling organoid thickness is useful for achieving high cell density culture and the maintenance of hepatocyte function to avoid cell death in the core of the organoids due to limitation of oxygen supply. The bioreactor may be useful for the development of various applications using cultured hepatocytes.

Keywords
Primary hepatocyte culture; Three dimensional culture; Tissue engineering; Organoid; Bioreactor; Bioartificial liver; Oxygen transfer
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Evaluation of a bioreactor with stacked sheet shaped organoids of primary hepatocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 5, May 2009, Pages 552–555
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us