fulltext.study @t Gmail

Isolation and structural characterization of a novel polysaccharide prepared from Arca subcrenata Lischke

Paper ID Volume ID Publish Year Pages File Format Full-Text
22195 43260 2007 6 PDF Available
Title
Isolation and structural characterization of a novel polysaccharide prepared from Arca subcrenata Lischke
Abstract

A water-soluble polysaccharide was isolated from Arca subcrenata Lischke (named ASLP) by hot-water extraction, anion-exchange, and gel-permeation chromatography. The average molecular weight of ASLP was estimated to be 3500 Da. The structural characterization of ASLP was performed by sugar composition analysis, methylation analysis, and partial acidic hydrolysis. Further analysis of ASLP was carried out by UV, FT-IR and NMR spectroscopies (1D, COSY, and HSQC, respectively). Our data suggests that ASLP is an α-(1→4)-D-glucan, with an α-(1→6)-D-glucan at the C-6 position every fourth residue along the main chain. The branch chain has three glucose residues. The possible structure, determined on the basis of structural analyses results, was also determined. Preliminary in vitro tests revealed that ASLP can stimulate mouse spleen lymphocyte proliferation and its branches are extremely important for its immunological activity.

Keywords
Arca subcrenata Lischke; polysaccharide; gas liquid chromatograph/mass spectrometry; nuclear magnetic resonance; structural characterization; spleen lymphocyte proliferation
First Page Preview
Isolation and structural characterization of a novel polysaccharide prepared from Arca subcrenata Lischke
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 2, August 2007, Pages 111–116
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering