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Biophysical studies on collagen-lipid interaction

Paper ID Volume ID Publish Year Pages File Format Full-Text
22200 43260 2007 5 PDF Available
Title
Biophysical studies on collagen-lipid interaction
Abstract

The potential use of liposomes as a delivery system is still limited by the poor understanding of the interaction mechanisms of liposomes underlying with biological media. Interaction between liposomes and protein is important for the structure and function of cells. In the present work, the interaction between collagen and dipalmitoyl phosphatidylcholine (DPPC) liposomes was studied by solubilization using a nonionic detergent, octylglucoside (OG), as well as a monolayer technique. The solubilization of the liposomal membrane was found to proceed in three stages of transition from the vesicular form to the mixed micellar form. Moreover, the amount of detergent needed to completely solubilize the liposomal membrane was increased after the incubation of liposomes with collagen, indicating an increased membrane resistance to the detergent and hence, a change in the natural membrane permeation properties. The addition of collagen in the subphase of different monolayer films induced a considerable shift towards a larger area/molecule in the compression-isotherm curves. This is either due to the insertion of collagen into the monolayer via its hydrophobic residues or to adsorption causing a protein layer to be located parallel to the lipid monolayer. It was concluded that collagen significantly altered the physical state of the liposome membrane, which may be attributed to collagen interaction with the liposomal surface and/or to its incorporation within the bilayer membrane.

Keywords
collagen; liposome; dipalmitoyl phosphatidylcholine; solubilization; monolayer
First Page Preview
Biophysical studies on collagen-lipid interaction
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 2, August 2007, Pages 144–148
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering