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Reverse transfection using antibodies against a cell surface antigen in mammalian adherent cell lines

Paper ID Volume ID Publish Year Pages File Format Full-Text
22202 43260 2007 4 PDF Available
Title
Reverse transfection using antibodies against a cell surface antigen in mammalian adherent cell lines
Abstract

Reverse transfection from a solid surface has the potential to deliver genes to various cells more efficiently than conventional methods. However, the effective gene delivery from a solid surface requires an optimized extracellular matrix (ECM) for the coating of glass slides, dependent on the nature of the cells. In a search for an appropriate substrate for the universal application to multiple types of cell, we focused on cell surface antigens and examined the effects of antibodies raised against them on gene transfer from an antibody-coated surface. We found that a coating of CD29-specific antibody allowed the most effective delivery of genes by reverse transfection in every type of cell that we examined. Our results suggest that reverse transfection with antibodies against CD29 might provide a universal tool for gene delivery and cell array-based analyses.

Keywords
reverse transfection; extracellular matrix; cell surface antigen; integrin; antibody
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Reverse transfection using antibodies against a cell surface antigen in mammalian adherent cell lines
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 2, August 2007, Pages 152–155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us