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Cynomolgus monkey embryonic stem cell lines express green fluorescent protein

Paper ID Volume ID Publish Year Pages File Format Full-Text
22205 43261 2006 7 PDF Available
Title
Cynomolgus monkey embryonic stem cell lines express green fluorescent protein
Abstract

We successfully established cynomolgus monkey embryonic stem (cES) cell lines expressing enhanced green fluorescent protein (GFP) by introducing a GFP-encoding gene under cytomegalovirus immediate early enhancer (CMVIE) promoter regulation into cES cells. The cells maintained the ability of in vitro differentiation toward ectodermal, mesodermal, and endodermal lineages, and produced teratomas composed of tissues derived from the three embryonic germ layers when transplanted into severe combined immunodeficient disease mice. GFP expression was also observed in the differentiated cells. These GFP-expressing cES cell lines are considered useful for basic research, including cell transplantation.

Keywords
primate embryonic stem cell; differentiation; embryoid body; teratoma; green fluorescent protein
First Page Preview
Cynomolgus monkey embryonic stem cell lines express green fluorescent protein
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 1, July 2006, Pages 14–20
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering