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Microbial transformation of aniline derivatives: Regioselective biotransformation and detoxification of 2-phenylenediamine by Bacillus cereus strain PDa-1

Paper ID Volume ID Publish Year Pages File Format Full-Text
22206 43261 2006 7 PDF Available
Title
Microbial transformation of aniline derivatives: Regioselective biotransformation and detoxification of 2-phenylenediamine by Bacillus cereus strain PDa-1
Abstract

A bacterial isolate, strain PDa-1, grew well on basal medium supplemented with 2-phenylenediamine, sucrose, and ammonium nitrate and completely transformed 2-phenylenediamine. The isolate was identified as Bacillus cereus. The product formed from 2-phenylenediamine was identified by EI-MS and NMR as 2-aminoacetanilide; whole cells converted 2-phenylenediamine to the product with a 76% molar yield. Whole cells also showed a broad substrate specificity toward 20 of 26 tested arylamines with substituent groups of various size and positions. Especially 2-aminobenzoic acid, 4-aminosalicylic acid, 5-aminosalicylic acid, and 2-aminofluorene were converted completely to the corresponding product with an aminoacetyl group. Cell extracts of strain PDa-1 had a high arylamine N-acetyltransferase activity. The partially purified enzyme converted 2-phenylenediamine to 2-aminoacetanilide. Strain PDa-1 constitutively expressed the enzyme in the absence of 2-phenylenediamine. Effects of 2-phenylenediamine and 2-aminoacetanilide on growth indicated that this enzyme probably plays a role in the detoxification of toxic arylamines in this strain.

Keywords
Bacillus cereus; 2-phenylenediamine; 2-aminoacetanilide; arylamine; arylamine N-acetyltransferase
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Microbial transformation of aniline derivatives: Regioselective biotransformation and detoxification of 2-phenylenediamine by Bacillus cereus strain PDa-1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 1, July 2006, Pages 21–27
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us