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Performance of expression vector, pTD1, in insect cell-free translation system

Paper ID Volume ID Publish Year Pages File Format Full-Text
22214 43261 2006 3 PDF Available
Title
Performance of expression vector, pTD1, in insect cell-free translation system
Abstract

We constructed a pTD1 vector for an insect cell-free translation system containing a 5′ untranslated region (UTR) of a polyhedrin gene as a translational enhancer sequence. Its translational efficiency was about 50-fold higher than those of mRNAs without an enhancer sequence. Moreover, the pTD1 vector functioned as an effective expression vector not only in the insect cell-free translation system but also in wheat germ extract and rabbit reticulocyte lysate systems.

Keywords
translational enhancer sequence; cell-free translation system; baculovirus polyhedrin; 5′ untranslated region
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Performance of expression vector, pTD1, in insect cell-free translation system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 1, July 2006, Pages 69–71
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us