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Double transfection into primary dissociated neurons

Paper ID Volume ID Publish Year Pages File Format Full-Text
22232 43262 2007 3 PDF Available
Title
Double transfection into primary dissociated neurons
Abstract

Synapses play a fundamental role in the processing of information in a neuronal network by mediating communication between neurons. To monitor presynaptic and postsynaptic structures simultaneously in living neurons, we developed double transfection method on the basis of calcium phosphate coprecipitation and introduced two fluorescent reporters into cultured neurons. The optimized protocol allowed different populations of neurons to be visualized by different color fluorescences, and presynaptic and postsynaptic structures to be observed in the same field of view of a microscope.

Keywords
transfection; calcium phosphate coprecipitation; hippocampal neurons; primary neuron culture; fluorescent protein
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Double transfection into primary dissociated neurons
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 103, Issue 5, May 2007, Pages 497–499
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us