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Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
22254 43264 2009 4 PDF Available
Title
Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli
Abstract

Allophycocyanin is a phycobiliprotein with various biological and pharmacological properties. An expression vector was constructed using CpeS as the bilin lyase for the allophycocyanin β subunit, resulting in overexpression of a fluorescent allophycocyanin β-subunit in Escherichia coli. A high-density cell culture was developed using a continuous feeding strategy. After 16 h of culture, the dry cell density reached 21.4 g l− 1, the expression of the allophycocyanin β-subunit was 0.86 g l− 1 broth, and the relative chromoprotein yield was 81.4%. The recombinant protein showed spectral features similar to native allophycocyanin, which provide an efficient methodology for large-scale production of this valuable fluorescent protein.

Keywords
ApcA, allophycocyanin α subunit; ApcB, allophycocyanin β subunit, apoproteins of ApcB are encoded by apcB; CpcB, phycocyanin β subunit; CpeS, phycocyanin β-subunit phycocyanobilin lyase; holo-ApcB, apo-ApcB with PCB; Hox1, heme oxygenase 1; HT, His6-tag;
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Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 3, March 2009, Pages 246–249
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us