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Stimulating effects of fibroblast growth factors on hepatic function of fetal liver cells synergistically with oncostatin M in three-dimensional culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
22266 43264 2009 5 PDF Available
Title
Stimulating effects of fibroblast growth factors on hepatic function of fetal liver cells synergistically with oncostatin M in three-dimensional culture
Abstract

Fetal liver cells (FLCs) are regarded as a feasible cell source of bioartificial liver (BAL), because the FLCs have proliferating ability even in vitro. However, the cellular functions of FLCs are considerably lower compared with mature hepatocytes. Thus, maturation of cultured FLCs is essential for enhancing the performance of the BAL using the FLCs. In the present study, the effects of fibroblast growth factors (FGF-1, FGF-2, and FGF-4) on cell growth and the liver-specific functions of mouse FLCs were investigated in the presence or absence of oncostatin M (OSM), under both three-dimensional (3-D) and monolayer culture conditions. When FGF-2 was used, no stimulating effects on the albumin secretion activities of the FLCs were observed either in the 3-D or monolayer cultures, although cell growth was improved in these cultures. In the cases of FGF-1 and FGF-4, these factors also had no effect on the albumin secretion activities in the absence of OSM. However, in the presence of OSM, FGF-1 and FGF-4 significantly enhanced the activities of the FLCs but only in the 3-D cultures. From scanning electron microscopic observation, the 3-D culture FLCs formed big cell aggregates on the surface of a porous scaffold. In conclusion, it was clarified that FGF-1 and FGF-4 facilitate the maturation of 3-D culture FLCs synergistically with OSM.

Keywords
Bioartificial liver; Fetal liver cell; Fibroblast growth factor; Oncostatin M; Three-dimensional culture; Tissue engineering; Porous scaffold
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Stimulating effects of fibroblast growth factors on hepatic function of fetal liver cells synergistically with oncostatin M in three-dimensional culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 3, March 2009, Pages 307–311
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us