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Bone regeneration using collagen type I vitrigel with bone morphogenetic protein-2

Paper ID Volume ID Publish Year Pages File Format Full-Text
22268 43264 2009 6 PDF Available
Title
Bone regeneration using collagen type I vitrigel with bone morphogenetic protein-2
Abstract

Bone morphogenetic protein-2 is a very promising candidate for the treatment of bone diseases and defects, but more effective therapeutic methods are required due to its instability in vivo. A controlled and localized delivery system of Bone morphogenetic protein-2 would be appropriate for effective bone regeneration. Here, we report a novel delivery system of bone morphogenetic protein-2 using vitrigel (a novel stable collagen gel membrane prepared from vitrified type I collagen) for in vivo bone regeneration. Scanning electron microscopy revealed that the collagen vitrigel formed a tightly woven network with average pore sizes of about 1–2 μm. The vitrigel scaffold delivery system exhibited sustained release of bone morphogenetic protein-2 and > 80% of the total bone morphogenetic protein-2 was still retained in the vitrigel after 15 days in phosphate-buffered saline in vitro. Bone morphogenetic protein-2-containing vitrigel was transplanted into mouse calvarial defects. The enhanced mechanical strength of the vitrigel made it easier to implant into defects without damage. Obvious bone regeneration was observed in the defects of mice treated with as little as 0.19 μg of bone morphogenetic protein-2 at 4 weeks after the transplantation. The local and sustained delivery system for bone morphogenetic protein-2 developed in the present study may represent a powerful modality for bone regeneration.

Keywords
Bone morphogenetic protein; Bone regeneration; Vitrigel; Scaffold; Sustained delivery
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Bone regeneration using collagen type I vitrigel with bone morphogenetic protein-2
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 3, March 2009, Pages 318–323
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us