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Development of peptide arrays for detection of IgE-binding epitopes in cow's milk allergens

Paper ID Volume ID Publish Year Pages File Format Full-Text
22269 43264 2009 7 PDF Available
Title
Development of peptide arrays for detection of IgE-binding epitopes in cow's milk allergens
Abstract

Peptide arrays have become versatile tools for high throughput screening assays in biomedical and pharmaceutical research. In this study, we constructed a peptide array that contained linear peptide fragments reported as IgE-binding epitopes for cow's milk allergy (CMA). Various peptides with different solubility in aqueous solutions were dissolved in the buffer solutions containing sodium dodecyl sulfate, and we achieved a consistent spotting of peptide solutions using a piezoelectric ceramic micropump. The IgE-binding patterns were successfully detected by observing the binding of Alexa 647-labeled anti-human IgE using sera from CMA patients. Our technique in this study will provide a potent capability for the development of a peptide array for mapping IgE-epitopes in milk proteins, and it will help researchers better understand the IgE-epitopes associated with the clinical outcome of CMA.

Keywords
Peptide array; Piezoelectric ceramic micropump; Cow's milk allergy; IgE-binding epitopes; spotting buffer
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Development of peptide arrays for detection of IgE-binding epitopes in cow's milk allergens
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 3, March 2009, Pages 324–330
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us