fulltext.study @t Gmail

Electrically induced protein release from Schizosaccharomyces pombe cells in a hyperosmotic condition during and following a high electropulsation

Paper ID Volume ID Publish Year Pages File Format Full-Text
22274 43265 2007 5 PDF Available
Title
Electrically induced protein release from Schizosaccharomyces pombe cells in a hyperosmotic condition during and following a high electropulsation
Abstract

A high-electric-field application of fission yeast cells under hyperosmotic conditions improved the electro-induced release of protein and cytoplasmic enzymes. A cell suspension was pulsed at 12.5 kV/cm for 10 ms in a batch system and immediately postincubated at 30°C. The total protein release rate from cells increased in the hypertonic solutions of 1.5–2.5 M sorbitol to approximately threefold higher than those in the hypotonic and isotonic solutions of 0–0.5 M sorbitol. The protein release rate in 2.0 M sorbitol greatly increased up to 4.5 h and then gradually increased after 7.5 h. The maximum activities of cytoplasmic enzymes, such as alcohol dehydrogenase and 3-phosphoglycerate kinase, were obtained at 7.5 h after electropulsation in 2.0 M sorbitol, yielding approximately 90% of the enzyme activity levels found in spheroplast lysate. On the other hand, the release rates of protease in vacuoles and invertase in periplasmic space did not depend on osmotic solutions during electric pulse application.

Keywords
electroextraction; osmolarity; enzyme release; Schizosaccharomyces pombe
First Page Preview
Electrically induced protein release from Schizosaccharomyces pombe cells in a hyperosmotic condition during and following a high electropulsation
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 103, Issue 4, April 2007, Pages 298–302
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us