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Image cytometry for analyzing regional distribution of cells inside human neurospheres

Paper ID Volume ID Publish Year Pages File Format Full-Text
22288 43265 2007 4 PDF Available
Title
Image cytometry for analyzing regional distribution of cells inside human neurospheres
Abstract

An image cytometry algorithm was developed to identify and quantify immunologically labeled cells in cultured human neurospheres. Image cytometry revealed new findings in terms of the localization of specific types of neural cells and the regional fluctuation of cell density in neurospheres. The distributions of total cell density exhibited similar profiles along the radial distance from the periphery of a neurosphere, irrespective of neurosphere sizes examined; approximately 1.0×106 cell/mm3 in the vicinity of the periphery and 6.0×105 cell/mm3 in an inner region of 50–180 μm. In addition, BrdU-positive (proliferating) cells were present at ratios of 53–64% across the entire region of the neurospheres, whereas BrdU-negative and β III tubulin-positive cells (postmitotic neurons) were minorities in the neurospheres.

Keywords
neural stem/progenitor cells; neurosphere; regional cell distribution; image cytometry
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 103, Issue 4, April 2007, Pages 384–387
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us