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Molecular cloning and functional analysis of minD gene from streptomyces lavendulae ATCC25233

Paper ID Volume ID Publish Year Pages File Format Full-Text
22304 43267 2008 3 PDF Available
Title
Molecular cloning and functional analysis of minD gene from streptomyces lavendulae ATCC25233
Abstract

The minD gene encodes an inhibitor of cell division, which is involved in the regulation of the placement of the proper cell division site in rod-shaped bacteria. In the present study, we cloned the MinD homolog from the filamentous bacterium Streptomyces lavendulae ATCC25233. We showed that the protein exhibits ATPase activity and gives rise to a filamentous phenotype in Escherichia coli. However, the introduction of minD into Streptomyces lividans gave no apparent morphological alternation. We purified the Streptomyces minD gene product, expressed it in E. coli, and characterized it by enzyme kinetics. This is the first report characterizing the Streptomyces MinD homolog.

Keywords
cell division inhibitor; morphological change; ATPase; Streptomyces lavendulae
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Molecular cloning and functional analysis of minD gene from streptomyces lavendulae ATCC25233
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 3, September 2008, Pages 303–305
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us