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N-linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
22309 43268 2006 7 PDF Available
Title
N-linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells
Abstract

cDNA encoding H- and L-chains from a mouse monoclonal antibody was introduced into tobacco BY2 cells, and the resulting sugar chain structures of plant-produced antibodies were analyzed by a combination of HPLC, exoglucosidase digestion and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The glycan structures determined were Man5–6GlcNAc2 (22.3%), GlcNAcMan5GlcNAc2 (3.1%), GlcNAcMan3FucXylGlcNAc2 (24.4%), GlcNAcMan3XylGlcNAc2 (17.8%), Man3FucXylGlcNAc2 (24.3%), and Man3XylGlcNAc2 (8.1%). The major glycan structures of the antibodies produced by transgenic suspension-cultured cells contain typical plant bisecting β(1 and 2)-xylose and α(1 and 3)-fucose residues, suggesting the posttranslational modification of a recombinant antibody in the late Golgi apparatus.

Keywords
IgG; tobacco; glycosylation; oligosaccharide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 3, March 2006, Pages 212–218
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us