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Quantitative expression analysis of inulinase gene cluster of Penicillium sp. strain TN-88

Paper ID Volume ID Publish Year Pages File Format Full-Text
22321 43268 2006 3 PDF Available
Title
Quantitative expression analysis of inulinase gene cluster of Penicillium sp. strain TN-88
Abstract

The filamentous fungus Penicillium sp. strain TN-88 carries the endoinulinase gene inuC and the exoinulinase gene inuD that are linked head-to-head on the genome and divergently transcribed from an 859-bp intergenic region [Moriyama et al., Biosci. Biotechnol. Biochem., 66, 1887–1896 (2002)]. Quantitative real-time PCR amplification revealed that the transcription levels of the inuC and inuD genes increased 42- and 3260-fold in inulin-grown mycelia, respectively. Sucrose as well as fructose did not induce the expression of the inuC or inuD gene at all. The levels of inuC and inuD transcripts in mycelia grown on the glucose/inulin mixture were both below their basal levels in glucose-grown mycelia. Thus, glucose exerts a strong carbon catabolite repression on the expression of the two genes.

Keywords
gene cluster; endoinulinase; exoinulinase; divergent transcription; intergenic region; inulin; Penicillium sp.; quantitative real-time PCR
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Quantitative expression analysis of inulinase gene cluster of Penicillium sp. strain TN-88
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 3, March 2006, Pages 277–279
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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