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Response of human epithelial cells to culture surfaces with varied roughnesses prepared by immobilizing dendrimers with/without d-glucose display

Paper ID Volume ID Publish Year Pages File Format Full-Text
22337 43269 2007 8 PDF Available
Title
Response of human epithelial cells to culture surfaces with varied roughnesses prepared by immobilizing dendrimers with/without d-glucose display
Abstract

To investigate the response of human epithelial cells to substrates with nanoscale modifications, dendrimer-immobilized surfaces were prepared with or without d-glucose displayed as a terminal ligand, giving topographic structures with mean roughnesses (Ra) of 1.8–11.0 nm. With an increase in the Ra value up to 4.0 nm, the epithelial cells cultured on naked dendrimer surface without d-glucose display were somewhat stretched in their morphology compared with those on a nonmodified plain surface. However, for the Ra values higher than 4.0 nm, such cell stretching was inhibited, resulting in the predominant existence of round-shaped cells. The change in cell morphology was appreciable on the surfaces with d-glucose-displayed dendrimers. When the Ra value increased up to 4.5 nm on these surfaces, in particular, the enhancement of cell stretching was recognized, and fluorescence microscopic observation supported the hypothesis that the glucose-transporter-mediated adhesion of cells to the surface encouraged the development of filopodia and stress fibers, thereby improving focal contact with the surface. Our results suggest that the combination of displaying d-glucose and modulating roughness can promote cytoskeletal formation accompanied by marked cell elongation on culture surfaces.

Keywords
human epithelial cells; dendrimer-immobilized surface; glucose display; nano-scale roughness; cell roundness; cytoskeleton; glucose transporter
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Response of human epithelial cells to culture surfaces with varied roughnesses prepared by immobilizing dendrimers with/without d-glucose display
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 103, Issue 2, February 2007, Pages 192–199
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us