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Effect of amplification of desensitized purF and prs on inosine accumulation in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
22347 43270 2007 7 PDF Available
Title
Effect of amplification of desensitized purF and prs on inosine accumulation in Escherichia coli
Abstract

The effect of a phosphoribosylpyrophosphate (PRPP) synthetase gene (prs) that was desensitized to feedback inhibition by ADP on inosine accumulation was investigated using an inosine-producing mutant of Escherichia coli. At the same time, various types of plasmid having a PRPP amidotransferase gene (purF) that was desensitized to feedback inhibition by AMP and GMP were also investigated to improve inosine productivity using a compatible plasmid containing prs with a plasmid containing purF. The recombinant E. coli I-9 harboring a low-copy-number plasmid having the desensitized-purF (pMWKQ) accumulated 3.6 g/l inosine from 40 g/l glucose in a 2-d culture. Furthermore, desensitized-prs amplification, in addition to purF, resulted in the accumulation of 6.2 g/l inosine. Additionally, through these experiments, a spontaneous mutant with an enhanced inosine-producing ability compared with the parent strain I-9 was obtained. The spontaneous mutant I-9m harboring only pMWKQ and I-9m harboring both pMWKQ and pSTVDA (a plasmid having the desensitized-prs) accumulated 6.7 g/l and 7.5 g/l inosine, respectively, from 40 g/l glucose in a 3-d culture.

Keywords
prs; purF; inosine accumulation; Escherichia coliE-4-P, erythrose-4-phosphate; S-7-P, sedoheptulose-7-phosphate
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Effect of amplification of desensitized purF and prs on inosine accumulation in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 103, Issue 3, March 2007, Pages 255–261
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us