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Innovative metabolic pathway design for efficient l-glutamate production by suppressing CO2 emission

Paper ID Volume ID Publish Year Pages File Format Full-Text
22348 43270 2007 8 PDF Available
Title
Innovative metabolic pathway design for efficient l-glutamate production by suppressing CO2 emission
Abstract

In the pathway of l-glutamic acid (l-Glu) biosynthesis in Corynebacterium glutamicum, 1 mol of l-Glu is synthesized from 1 mol of glucose at a cost of 1 mol of carbon dioxide (CO2), with a maximum theoretical yield of 81.7% by weight. We have designed an innovative pathway for efficient l-Glu production employing phosphoketolase (PKT) to bypass the CO2-releasing pyruvate dehydrogenase reaction, thereby increasing the maximum theoretical yield of l-Glu from glucose to up to 98.0% by weight (120% mol/mol l-Glu produced/glucose consumed). The xfp gene encoding PKT was cloned from Bifidobacterium animalis and overexpressed under the strong cspB promoter in C. glutamicum. A functional enzyme was detected in an l-Glu-producing strain of C. glutamicum (odhA). When cells of this producer strain with the xfp gene and those without the xfp gene were cultivated in a controlled fermentation system, the l-Glu production yield of the strain expressing the xfp gene was much higher than that of the original strain, coupled with the suppression of CO2 emission. Consequently, we could successfully enhance l-glutamate production by installing the PKT pathway of B. animalis into C. glutamicuml-Glu metabolism, and this novel metabolic design will be able to increase l-Glu production yield beyond the maximum theoretical yield obtained from the conventional metabolic pathway of biosynthesis from glucose.

Keywords
l-glutamate production; phosphoketolase; metabolic engineering; Corynebacterium glutamicum; theoretical production yield; carbon dioxide; bifidobacterium
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Innovative metabolic pathway design for efficient l-glutamate production by suppressing CO2 emission
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 103, Issue 3, March 2007, Pages 262–269
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us