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High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
22367 43271 2007 3 PDF Available
Title
High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells
Abstract

The effects of the density of human mesenchymal stem cells (MSCs) on their differentioation to chondrocytes in a differentiation medium supplemented with dexamethasone, TGFβ3, and IGF-1 were investigated for the regenerative therapy of cartilage. The increase in the initial density of MSCs from 0.05×104 to 0.9×104 cells/cm2 accelerated the increase in the expression level of aggrecan mRNA during the differentiation culture for 7 d. The conditioned medium harvested at 7 d from the differentiation culture with an initial MSC density of 0.3×104 cells/cm2 accelerated the initial increase in the expression level for 3 d in the differentiation culture with an initial MSC density of 0.3×104 cells/cm2, whereas the conditioned medium harvested at 7 d in the differentiation culture with an initial MSC density of 0.05×104 cells/cm2 did not. The differentiation culture after 14 d with an initial MSC concentration of 0.3×104 cells/cm2 showed an expression level 1.7-fold that in the case of the culture with an initial MSC concentration of 0.05×104 cells/cm2. Thus, a high MSC inoculum density might be appropriate for the rapid differentiation of MSCs to chondrocytes.

Keywords
cell density; mesenchymal stem cell; differentiation; chondrocyte
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High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 103, Issue 1, January 2007, Pages 98–100
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us