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Kinetics of endoglucanase and endoxylanase uptake by soybean seeds

Paper ID Volume ID Publish Year Pages File Format Full-Text
22372 43273 2006 9 PDF Available
Title
Kinetics of endoglucanase and endoxylanase uptake by soybean seeds
Abstract

The uptakes of endoglucanase and endoxylanase by soybean were studied by measuring the fluorescent intensity of a carboxyfluorescein. The disappearance rates of these enzymes from solution were compared with predictions from different models. The Freundlich model provided the best fit for our data. Enzyme concentration in the solution did not change significantly after 11 d, and therefore it was assumed that equilibrium was reached. Average mass transfer coefficient was calculated for both endoglucanase and endoxylanase from the plot of the rate of uptake against (Cl av −Cl*) and a value of 4×10−5 ms−1 was obtained. K value was used to calculate the effective diffusivities of the two enzymes assuming a slice. It is found that K was asymptotic with long residence times, and a value of 4.8×10−10 m2 s−1 was obtained.

Keywords
endoglucanase; endoxylanase; soybean seeds; uptake kinetics; mass transfer coefficient
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Kinetics of endoglucanase and endoxylanase uptake by soybean seeds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 2, February 2006, Pages 111–119
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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