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Improved l-threonine production of Escherichia coli mutant by optimization of culture conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
22374 43273 2006 4 PDF Available
Title
Improved l-threonine production of Escherichia coli mutant by optimization of culture conditions
Abstract

l-Threonine production was investigated in a minimal salt medium using l-threonine-overproducing Escherichia coli MT201, derived from E. coli K-12. It was observed that dry cell weight reached 12.5 g/l with 15.9 g/ll-threonine. To increase dry cell weight and l-threonine production, the fermentation process was optimized. When biotin was added as growth factor, l-threonine production reached 52.0 g/l from 15.9 g/l without biotin. Dry cell weight and l-threonine production were further increased by continuous feeding of the feed media with an optimized l-methionine concentration (5.0 g/l). However, high-cell-density culture caused oxygen-limited condition, which resulted in the accumulation of organic acids. To overcome this problem, oxygen-enriched air was supplied to the fermentor with the minimal salt medium. Under these optimal conditions, we achieved an l-threonine production of 80.2 g/l in the minimal salt medium.

Keywords
l-threonine; Escherichia coli MT201; minimal salt medium; biotin; l-methionine
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Improved l-threonine production of Escherichia coli mutant by optimization of culture conditions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 2, February 2006, Pages 127–130
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us