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Oxalate enhances protein synthesis in cell-free synthesis system utilizing 3-phosphoglycerate as energy source

Paper ID Volume ID Publish Year Pages File Format Full-Text
22380 43273 2006 4 PDF Available
Title
Oxalate enhances protein synthesis in cell-free synthesis system utilizing 3-phosphoglycerate as energy source
Abstract

Herein, we report our results showing that the productivity of cell-free protein synthesis can be enhanced through the regulation of the in vitro metabolism of an energy source. In a reaction mixture utilizing 3-phosphoglycerate (3PG) as an energy source, the supply of ATP was significantly enhanced when the reaction mixture was supplied with sodium oxalate, a potent inhibitor of phosphoenolpyruvate synthetase (PPS). The productivity of protein synthesis was also increased by approximately 70% upon the addition of oxalate. It was presumed that this enhancement in ATP supply resulted from the prevention of the pyruvate → PEP reaction, which causes nonproductive ATP consumption. For the initial presence of 2.1 mM sodium oxalate, approximately 720 μg/ml chloramphenicol acetyltransferase (CAT) was produced after 3 h of incubation at 37°C.

Keywords
3-phosphoglycerate; glycolysis; ATP regeneration; pyruvate; phosphoenolpyruvate; cell-free protein synthesis; chloramphenicol acetyltransferase
First Page Preview
Oxalate enhances protein synthesis in cell-free synthesis system utilizing 3-phosphoglycerate as energy source
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 2, February 2006, Pages 162–165
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering