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Supplementation effects of hydroxyectoine on proline uptake of downshocked Brevibacterium sp. JCM 6894

Paper ID Volume ID Publish Year Pages File Format Full-Text
22383 43273 2006 7 PDF Available
Title
Supplementation effects of hydroxyectoine on proline uptake of downshocked Brevibacterium sp. JCM 6894
Abstract

Downshock treatment of the halotolerant Brevibacterium sp. JCM 6894 was a prerequisite for proline uptake which is a function for cell survival. Hydroxyectoine served as an effective stimulator for the proline uptake and cell survival of the downshocked cells of this strain. Duration of osmotic downshock, downshock strength, and the kinds of osmolyte affected the efficient rate of growth (ERG) and the uptake of proline. A shorter duration of osmotic downshock, that is ≤1 h, induced higher ERG values and stimulated proline uptake, compared with those of non-downshocked cells when incubated with hydroxyectoine and proline. These activities, however, were gradually suppressed with the prolongation of the duration of osmotic downshock. When the cells grown in KCl-containing medium were subjected to downshock treatment for 1 h, their activities were enhanced more remarkably than those of cells grown in NaCl-containing medium.

Keywords
proline; hydroxyectoine; ectoine; Brevibacterium sp. JCM 6894; osmotic downshock
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Supplementation effects of hydroxyectoine on proline uptake of downshocked Brevibacterium sp. JCM 6894
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 2, February 2006, Pages 178–184
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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