fulltext.study @t Gmail

Isolation of dibenzofuran-degrading bacterium, Nocardioides sp. DF412, and characterization of its dibenzofuran degradation genes

Paper ID Volume ID Publish Year Pages File Format Full-Text
22394 43274 2008 8 PDF Available
Title
Isolation of dibenzofuran-degrading bacterium, Nocardioides sp. DF412, and characterization of its dibenzofuran degradation genes
Abstract

A dibenzofuran (DF)-degrading bacterium, Nocardioides sp. DF412, was isolated and its genes responsible for the degradation of DF were cloned and characterized. The dfdA1A2A3A4 gene cluster coding for a ring hydroxylating dioxygenase was located on a plasmid, and the dfdBC gene cluster coding for a ring-cleavage dioxygenase and a following hydrolase respectively was located on the chromosome. Reverse transcription-PCR analysis revealed that the transcription of both gene clusters was induced in the presence of DF. These two gene clusters also conferred the activity of each enzyme to a host strain. These results strongly suggest that the clusters are responsible for the degradation of DF in strain DF412.

Keywords
dibenzofuran; dioxin; biodegradation; ring-hydroxylating dioxygenase; ring-cleavage dioxygenase; hydrolase
First Page Preview
Isolation of dibenzofuran-degrading bacterium, Nocardioides sp. DF412, and characterization of its dibenzofuran degradation genes
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 6, June 2008, Pages 628–635
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us