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Increase in pladienolide D production rate using a Streptomyces strain overexpressing a cytochrome P450 gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
22397 43274 2008 6 PDF Available
Title
Increase in pladienolide D production rate using a Streptomyces strain overexpressing a cytochrome P450 gene
Abstract

Pladienolide B and its 16-hydroxylated derivative (pladienolide D) are novel 12-membered macrolides produced by Streptomyces platensis Mer-11107 showing strong in vitro and in vivo antitumor activity. While pladienolide B is mainly produced by this strain, pladienolide D is produced to a lesser extent. To facilitate the production of pladienolide D by biotransformation, we found that Streptomyces bungoensis A-1544 was able to hydroxylate pladienolide B at 16-position. We identified psmA from S. bungoensis A-1544, which encoded a pladienolide B 16-hydroxylase PsmA belonging to the CYP105 family of cytochrome P450. To increase the efficiency of pladienolide D production, we constructed recombinant S. bungoensis A-1544 overexpressing psmA and performed biotransformation of pladienolide B to pladienolide D. This biotransformation achieved a production level 15-fold higher than that using the control strain S. bungoensis A-1544/pIJ702.

Keywords
pladienolide; Streptomyces bungoensis A-1544; cytochrome P450 monooxygenase; bacterial P450-expression system; biotransformation
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Increase in pladienolide D production rate using a Streptomyces strain overexpressing a cytochrome P450 gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 6, June 2008, Pages 649–654
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us