fulltext.study @t Gmail

Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin

Paper ID Volume ID Publish Year Pages File Format Full-Text
22418 43276 2008 5 PDF Available
Title
Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin
Abstract

Fluorescence labeling of a cytokine at a specific site is required for observing cytokine-receptor interactions in living cells at the single-molecule level. Here, we demonstrated the C-terminus-specific fluorescence labeling of histidine-tagged thrombopoietin (TPO), a ligand for Mpl, with desthiobiotin-tagged fluorescent puromycin. Fluorescent TPO, purified by tandem affinity purification, stimulated the proliferation of Mpl-expressing cells. Within 10 min of its addition, fluorescent TPO was found to be diffusely distributed on the cell membranes of Mpl-expressing cells, and gradually accumulated to form fluorescent spots. This method is applicable for studying the spatial and temporal distributions of cytokines in individual living cells.

Keywords
affinity tag; cytokine; desthiobiotin tag; fluorescence labeling method; fluorescence microscopy; puromycin; tandem affinity purification
First Page Preview
Fluorescence labeling of a cytokine with desthiobiotin-tagged fluorescent puromycin
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 3, March 2008, Pages 238–242
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us