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In Planta production of immunogenic poliovirus peptide using tobacco mosaic virus-based vector system

Paper ID Volume ID Publish Year Pages File Format Full-Text
22432 43278 2006 5 PDF Available
Title
In Planta production of immunogenic poliovirus peptide using tobacco mosaic virus-based vector system
Abstract

The tobacco mosaic virus (TMV) provides an attractive means of producing foreign peptides in plants. In this study, a TMV-based vector was designed such that a fragment encoding 15 amino acids of the poliovirus peptide (PVP) derived from the viral capsid proteins VP3 and VP1 of poliovirus type 1 Sabin was inserted downstream of the six-base 3′ context nucleotide sequence of the TMV coat protein (CP) gene. This design allowed readthrough at the amber stop codon, thereby producing the chimeric TMV particle with both intact CP and CP-fusion protein (CP-PVP) in Nicotiana tabacum cv. Samsun infected with the TMV vector. The TMVCP-PVP virus particle induced antibodies against PVP as well as TMVCP in mice after intraperitoneal immunization. These data illustrate the potential of the readthrough translation system with TMVCP for antigen presentation and vaccine production.

Keywords
poliovirus; tobacco mosaic virus; Nicotiana tabacum cv. SamsunCP, coat protein; HRP, horseradish peroxidase; PVP, poliovirus peptide; TMV, tobacco mosaic virus; ToMV, tomato mosaic virus; VP, viral proteins
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In Planta production of immunogenic poliovirus peptide using tobacco mosaic virus-based vector system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 5, May 2006, Pages 398–402
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us