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Purification and Characterization of 2-Aminoacetophenone Reductase of Newly Isolated Burkholderia sp. YT

Paper ID Volume ID Publish Year Pages File Format Full-Text
22465 43288 2007 4 PDF Available
Title
Purification and Characterization of 2-Aminoacetophenone Reductase of Newly Isolated Burkholderia sp. YT
Abstract

We found that a newly isolated Burkholderia sp. produced (R)-2-amino-1-phenylethanol from 2-aminoacetophenone, showing the high stereospecificity. NADPH-dependent 2-aminoacetophenone reductase purified to homogeneity was a dimer with a molecular mass of 65,000. The purified enzyme did not reduce acetophenone and 1-phenyl-1-propanone. The purified enzyme converted 2-aminoacetophenone to only (R)-2-amino-1-phenylethanol.

Keywords
2-aminoacetophenone reductase; (R)-2-amino-1-phenylethanol production; Burkholderiasp.
First Page Preview
Purification and Characterization of 2-Aminoacetophenone Reductase of Newly Isolated Burkholderia sp. YT
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 5, 2007, Pages 416–419
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering