fulltext.study @t Gmail

Characterization of neurons differentiated from mouse embryonic stem cells using conditioned medium of dorsal root ganglia

Paper ID Volume ID Publish Year Pages File Format Full-Text
22473 43291 2007 6 PDF Available
Title
Characterization of neurons differentiated from mouse embryonic stem cells using conditioned medium of dorsal root ganglia
Abstract

Mouse embryonic stem (ES) cells have the pluripotent ability to differentiate in vitro into various cell lineages, including neurons. Adding chick dorsal root ganglion (DRG) conditioned medium (CM) to the culture medium promotes the differentiation of ES cells into neurons. We determined the types of neurons that differentiate from ES cells. The addition of DRG-CM caused nearly half of all ES cells on the periphery of the colony sphere to differentiate into neurons. Immunofluorescence analysis showed that the neurons that differentiated from ES cells were mainly motor, GABAergic, serotonergic, and cholinergic neurons. Of particular note, flow cytometry showed that approximately 50% of βIII-tubulin-positive neurons were motor neurons. This indicates that DRG-CM induces ES cells to differentiate into motor neurons as target of DRG neurons (sensory neurons).

Keywords
conditioned medium of dorsal root ganglia; differentiation; embryonic stem cell; motor neuronBrdU; 5-bromo-2′-deoxyuridine; ChAT; choline acetyltransferase; CM; conditioned medium; DBH; dopamine β-hydroxylase; DRG; dorsal root ganglion; ES; embryonic stem
First Page Preview
Characterization of neurons differentiated from mouse embryonic stem cells using conditioned medium of dorsal root ganglia
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 4, October 2007, Pages 257–262
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us