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Hyperproduction of sebaceous cis-6-hexadecenoic acid by esterase-reduced mutant of Rhodococcus sp. strain

Paper ID Volume ID Publish Year Pages File Format Full-Text
22480 43291 2007 4 PDF Available
Title
Hyperproduction of sebaceous cis-6-hexadecenoic acid by esterase-reduced mutant of Rhodococcus sp. strain
Abstract

cis-6-Hexadecenoic acid is a major component of human sebaceous lipids that is involved in skin self-sterilization and atopic dermatitis amelioration. It can be prepared by hydrolysis of isopropyl cis-6-hexadecenoate produced by resting cells of Rhodococcus sp. strain KSM-MT66. To devise an economical industrial-scale process for the production of this rare fatty acid, we optimized the conditions for growing rhodococcal cells. Mg2+ and Fe2+ ions are essential for the efficient production of isopropyl cis-6-hexadecenoate. To further increase the production of isopropyl cis-6-hexadecenoate, we created a mutant strain (T64) with reduced esterase activity by random mutagenesis using UV irradiation of MT66. Under an optimized condition, the mutant T64 produced more than 60 g lāˆ’1 isopropyl cis-6-hexadecenoate in a 4-d cultivation, corresponding to about 52 g lāˆ’1cis-6-hexadecenoate.

Keywords
cis-6-hexadecenoic acid; sebaceous lipid; Rhodococcus; atopic dermatitis
First Page Preview
Hyperproduction of sebaceous cis-6-hexadecenoic acid by esterase-reduced mutant of Rhodococcus sp. strain
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 4, October 2007, Pages 300ā€“303
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering