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Observational examination of aggregation and migration during early phase of neurosphere culture of mouse neural stem cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
22503 43295 2007 4 PDF Available
Title
Observational examination of aggregation and migration during early phase of neurosphere culture of mouse neural stem cells
Abstract

Neural stem cells (NSCs) form cell aggregates called neurospheres during serum-free culture, and this aggregation contributes primarily to neurosphere development. In the present study, we conducted an observational examination of the cell clustering of mouse NSCs in relation to their migration potential. The cell clustering became significant from 12 h after the inoculation of NSCs during the examined culture period of 48 h. Three types of clustering were observed, that is, aggregation between two single cells, and coalescence between a cell and an aggregate and that between two aggregates. The aggregation between single cells occurred during the early phase (0–24 h after inoculation). Six hours after inoculation, the average migration rate of single cells that resulted in aggregation within the subsequent 12 h was higher than that of cells that did not aggregate. Fluorescent stainings of the cytoplasm and F-actin revealed the existence of mini podia and long podia, which were possible elements that produce the driving force for the migration of singly occurring NSCs.

Keywords
neural stem cells; neurosphere formation; aggregation; cell migration
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Observational examination of aggregation and migration during early phase of neurosphere culture of mouse neural stem cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 3, September 2007, Pages 231–234
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us