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Establishment of ovarian reconstruction system in culture for functional genomic analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
22510 43300 2006 6 PDF Available
Title
Establishment of ovarian reconstruction system in culture for functional genomic analysis
Abstract

In a previous study, we reported a list of genes expressed differentially in primordial and primary follicles [Park et al., Fertil. Steril., 83, 410–418 (2005)]. An innovative experimental system is required to evaluate the functions of these genes in folliculogenesis, particularly primordial-primary follicle transition. In this study, ovarian tissues were dissociated, and isolated cells were transfected using small interfering RNAs (siRNAs) for disrupting a specific gene, followed by ovarian reconstruction via calcium alginate encapsulation. The effects of RNA interference (RNAi) on follicular development were evaluated by a histological observation of the reconstructed ovarian tissue. Interestingly, follicular formation and development showed differences between control and experimental groups. Thus, even though this system includes some problems that need to be solved, ovarian reconstruction following the modification of gene expression of individual ovarian component cells could lead the way to methods of studying molecular mechanisms of primordial-primary follicle transition.

Keywords
calcium alginate encapsulation; ovarian reconstruction; primordial-primary follicle transition; RNA interference (RNAi)
First Page Preview
Establishment of ovarian reconstruction system in culture for functional genomic analysis
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 5, November 2006, Pages 396–401
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering