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Determination of enzymes from Colletotrichum sp. AHU9748 essential for lepidimoide production from okra polysaccharide

Paper ID Volume ID Publish Year Pages File Format Full-Text
22518 43300 2006 5 PDF Available
Title
Determination of enzymes from Colletotrichum sp. AHU9748 essential for lepidimoide production from okra polysaccharide
Abstract

The allelopathic substance lepidimoide (Lp), which exhibits multiple functions in the growth and development of plants, was produced by Colletotrichum sp. AHU9748 from okra polysaccharide. Okra polysaccharide has the repeating structure (1→4)-O-α-(d-galactopyranosyluronic acid)-(1→2)-O-α-l-rhamnopyranose in its hexasaccharide repeating unit of its main chain. To determine the enzymes essential for Lp production, the supernatant of a culture broth was fractionated by repeated column chromatographies to identify two serial fractions responsible for Lp production and non-Lp production by measuring Lp production together with β-galactosidase (β-gal), rhamnogalacturonan lyase (RG-lyase) and acetylesterase (AE) activities, which we hypothesized to be necessary for Lp production from the structure of Lp. We confirmed the presence of these three enzymatic activities in the highest-Lp-producing fraction. The addition of purified RG-lyase to fractions producing no or a small amount of Lp demonstrated that β-gal and RG-lyase activities are necessary for Lp production. The N-terminal amino acid sequences of the three separated proteins on SDS–PAGE confirmed the presence of enzymes identical to β-gal, RG-lyase and AE in the Lp-producing fractions.

Keywords
lepidimoide; okra polysaccharide; endophytic fungi; Colletotrichum sp.; β-galactosidase; rhamnogalacturonan lyase; acetylesterase
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Determination of enzymes from Colletotrichum sp. AHU9748 essential for lepidimoide production from okra polysaccharide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 5, November 2006, Pages 452–456
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us